Biolegend, 631257, Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for Flow Cytometry) Antibody
25tests
Catalog Number: 631257
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Synthetic peptide from human c-Fos phosphorylated at Ser32. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | For ICFC use we recommend cell fixation/permeabilization with True-Phos™ Perm buffer. |
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| RRID | AB_3662313 (BioLegend Cat. No. 631257) AB_3662313 (BioLegend Cat. No. 631258) |
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| Structure | c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins. |
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| Distribution | Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus. |
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| Function | c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus. |
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| Interaction | c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor. |
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| Ligand/Receptor | As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription |
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| Biology Area | Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors |
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| Molecular Family | Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors |
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| Antigen References | Sasaki T, et al. 2006. Mol Cell. 24:63-75. Huang K, et al. 2014. Cell Signal. 26:629-38. Qu X, et al. 2019. Cancer Sci. 110:3183-3196. Guo JC, et al. 2015. PLoS One. 10:e0120332. Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818. |
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| Gene ID | 2353 |
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| UniProt | View information about c-Fos on UniProt.org |
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| Clone | A22009C |
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| Regulatory Status | RUO |
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| Other Names | G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1 |
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| Isotype | Mouse IgG2b, κ |
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