Biolegend, 639956, Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2) Antibody
100microg
Catalog Number: 639956
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Recombinant fragment of the intracellular domain of human CD340 (ErbB2/HER-2) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-P - Quality tested ICC, ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Additional Product Notes | For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended. For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol Fixation/permeabilization with 100% ice-cold methanol only is not recommended. |
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| RRID | AB_3720625 (BioLegend Cat. No. 639955) AB_3720625 (BioLegend Cat. No. 639956) |
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| Structure | CD340 is a 1255 amino acid protein with a predicted molecular weight of 137.9 kD. It is a glycosylated receptor-like transmembrane protein; due to glycosylation, the observed molecular weight by Western Blot is closer to 175-185 kD. |
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| Distribution | Cellular membrane and also present in internalized vesicles |
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| Function | Receptor, transcriptional regulator |
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| Interaction | Homodimerizes Heterodimerizes with EGFR, ErbB3, ErbB4, PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL |
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| Ligand/Receptor | Heterodimerizes with other ERBB family members upon ligand binding, but does not directly bind ligand |
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| Cell Type | Leukemia |
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| Biology Area | Cell Biology, Immunology, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukoc Biol. 74:593-601. |
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| Gene ID | 2064 |
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| UniProt | View information about CD340 on UniProt.org |
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| Clone | W22266D |
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| Regulatory Status | RUO |
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| Other Names | Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin |
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| Isotype | Rat IgG2a, κ |
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