Biolegend, 640454, Alexa Fluor® 647 anti-Rb Phospho (Ser795) Antibody
100tests
Catalog Number: 640454
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Synthetic peptide of human Rb phosphorylated at serine 795 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Additional Product Notes | For intracellular flow cytometry (ICFC) staining fixation and permeabilization we recommend use True-Phos™ Perm Buffer Set (Cat. No. 425401). Fixation/permeabilization using Cyto-Fast™ Fix/Perm buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) showed reduced mean fluorescence intensity (MFI). |
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| RRID | AB_3720506 (BioLegend Cat. No. 640453) AB_3720506 (BioLegend Cat. No. 640454) |
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| Structure | The Rb protein is 928 amino acids in length and has a molecular weight of approximately 106 kDa. |
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| Distribution | Nucleus, cytoplasmic during keratinocyte differentiation |
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| Function | • Tumor Suppression: Rb acts as a tumor suppressor by inhibiting excessive cell growth and proliferation. • Cell Cycle Regulation: Rb controls progression from the G1 phase to the S phase of the cell cycle by binding to and inhibiting E2F transcription factors. • Transcription Regulation: Rb regulates the transcription of genes necessary for DNA synthesis and cell cycle progression. • Apoptosis: Rb can also induce apoptosis under certain conditions, contributing to its role in preventing cancer. |
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| Interaction | • E2F Transcription Factors: Rb binds to E2F transcription factors, inhibiting their activity and preventing the transcription of genes required for cell cycle progression. • DP Proteins: Rb also interacts with DP proteins, which form heterodimers with E2F, enhancing the regulation of E2F activity. • Cyclin-Dependent Kinases (CDKs): CDKs phosphorylate Rb, leading to its inactivation and allowing the cell cycle to progress. • Histone Deacetylases (HDACs): Rb recruits HDACs to E2F target genes, leading to chromatin remodeling and transcriptional repression. • Chromatin Remodeling Enzymes: Rb interacts with various chromatin remodeling enzymes, such as methylases and acetylases, to regulate gene expression. |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors |
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| Molecular Family | Phospho-Proteins, Tumor Suppressors |
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| Antigen References | Rezaeian A, et al. 2023. Adv Protein Chem Struct Biol. 135:179. Janostiak R, et al. 2022. Cancers (Basel). 14:1265. Indovina P, et al. 2015. Oncotarget. 6:17873. Linn P, et al. 2021. Cancers (Basel). 13:3737. |
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| Regulation | • Phosphorylation: Rb is regulated by phosphorylation, particularly by cyclin-dependent kinases (CDKs). Phosphorylation at specific sites, such as serine 795, inactivates Rb, allowing cell cycle progression. • Dephosphorylation: Dephosphorylation of Rb reactivates its tumor suppressor functions, inhibiting cell cycle progression. • Gene Mutations: Mutations in the RB1 gene can lead to loss of Rb function, contributing to the development of cancers such as retinoblastoma, lung, bladder, and breast cancers. |
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| Gene ID | 5925 |
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| UniProt | View information about Rb Phospho Ser795 on UniProt.org |
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| Clone | A24001E |
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| Regulatory Status | RUO |
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| Other Names | RB1, Retinoblastoma-associated protein, p105-Rb, p110-RB1 |
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| Isotype | Mouse IgG1, κ |
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