Biolegend, 640904, Biotin Annexin V
100tests
Catalog Number: 640904
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| Verified Reactivity | Human, Mouse, Rat |
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| Reported Reactivity | Other Species |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Preparation | The purified protein was conjugated with biotin under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| Annexin V Staining Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL. Transfer 100 µL of cell suspension in 5 mL test tube. Add 5 µL of fluorochrome conjugated Annexin V. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step. Analyze by flow cytometry. For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents. |
| Application References(PubMed link indicates BioLegend citation) | Koopman G, et al. 1994. Blood 84:1415. Vermes I, et al. 1995. J. Immunol. Methods 184:39. Dachary-Prigent J, et al. 1993. Blood 81:2554. Sekine C, et al. 2009. Int. Immunol. 21:295. PubMed Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed Hussain MS, et al. 2013. Hum. Mol. Genet. 22:5199. PubMed Feng Q, et al. 2014. PLoS One. 9:95927. PubMed |
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| Product Citations | Dufour S, et al. 2023. Commun Biol. 6:207. PubMed Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed Richard A, et al. 2015. Proc Natl Acad Sci U S A. 112: 14682 - 14687. PubMed |
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| RRID | not an antibody (BioLegend Cat. No. 640904) not an antibody (BioLegend Cat. No. 640939) |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability, Neuroscience |
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| Gene ID | 308 |
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| UniProt | View information about Annexin V on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Annexin A5, Dead, Apoptosis |
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