Biolegend, 643804, Alexa Fluor® 594 anti-β-actin Antibody
100microg
Catalog Number: 643804
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality testedIHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes |
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| The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol. |
| Application References(PubMed link indicates BioLegend citation) | Lawson BR, et al. 2007. J. Immunol. 178:5366. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053. Yanagiya T,et al.2007.Obesity.15:572. KishidaT,et al. 2007.J. Immunol. 179:8554. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. Ikeda D, et al. 2008. Endocrinology. 149:6037. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed |
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| RRID | AB_2565573 (BioLegend Cat. No. 643804) |
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| Structure | Actin, cytoplasmic 1 |
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| Distribution | Ubiquitously expressed in the cytoplasm of all eukaryotic cells |
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| Function | Actins are highly conserved proteins that are involved in cell motility |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Microfilaments |
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| Antigen References | 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687. |
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| Gene ID | 11461 |
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| UniProt | View information about beta-actin on UniProt.org |
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| Clone | 2F1-1 |
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| Regulatory Status | RUO |
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| Other Names | Actin, cytoplasmic 1 |
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| Isotype | Mouse IgG2b, κ |
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