Biolegend, 644704, Alexa Fluor® 488 anti-GFAP Antibody
100microg
Catalog Number: 644704
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| Verified Reactivity | Human, Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Bovine spinal cord homogenate |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested ICC, IHC-P - Verified SB - Community verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.1 -10 µg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Additional Product Notes | This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page. |
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| Application References(PubMed link indicates BioLegend citation) | McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed |
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| Product Citations | Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed Körner A, et al. 2019. Nat Commun. 10:633. PubMed Ullah I, et al. 2021. Immunity. 54:2143. PubMed Ku T, et al. 2020. Nat Methods. 17:609. PubMed |
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| RRID | AB_2566109 (BioLegend Cat. No. 644704) |
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| Structure | Around 50 kD |
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| Distribution | Only expressed in astrocytes in the central nervous system. |
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| Function | Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein. |
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| Cell Type | Astrocytes |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Gene ID | 2670 |
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| UniProt | View information about GFAP on UniProt.org |
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| Clone | 2E1.E9 |
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| Regulatory Status | RUO |
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| Other Names | Glial fibrillary acid protein |
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| Isotype | Mouse IgG2b |
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