Biolegend, 644833, Alexa Fluor® 594 anti-T-bet Antibody
25microg
Catalog Number: 644833
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality testedICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml (1:100-1:250 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). |
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| Application References(PubMed link indicates BioLegend citation) | Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed. |
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| Product Citations | Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed |
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| RRID | AB_2728473 (BioLegend Cat. No. 644833) AB_2728474 (BioLegend Cat. No. 644834) |
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| Structure | T-box transcription factor, approximately 58 kD. |
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| Distribution | Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen. |
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| Function | Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens. |
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| Cell Type | B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907. |
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| Gene ID | 30009 |
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| UniProt | View information about T-bet on UniProt.org |
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| Clone | 4B10 |
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| Regulatory Status | RUO |
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| Other Names | T-box expressed in T cells, T box 21, TBLYM |
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| Isotype | Mouse IgG1, κ |
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