Biolegend, 647908, Alexa Fluor® 488 anti-Cyclin B1 Antibody
100tests
Catalog Number: 647908
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| Verified Reactivity | Human |
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| Reported Reactivity | Mouse |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Hamster Cyclin B1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips. |
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| Application References(PubMed link indicates BioLegend citation) | Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed Matsumura F, et al. 2011. J. Biol Chem. PubMed |
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| RRID | AB_2632639 (BioLegend Cat. No. 647907) AB_2632640 (BioLegend Cat. No. 647908) |
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| Structure | Belongs to the cyclin family. Cyclin AB subfamily. |
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| Distribution | Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD. |
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| Interaction | CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4 |
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| Bioactivity | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication |
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| Antigen References | 1. Morgan DO, et al.1995. Nature 374:131 |
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| Gene ID | 891 |
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| UniProt | View information about Cyclin B1 on UniProt.org |
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| Clone | V152 |
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| Regulatory Status | RUO |
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| Other Names | CCNB1 |
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| Isotype | Mouse IgG1 |
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