Biolegend, 649201, Purified anti-GAPDH Antibody
25microg
Catalog Number: 649201
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human CD4 lymphocytes |
| Formulation | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | Upon receipt, store undiluted between 2°C and 8°C. |
| Application | WB - Quality tested IHC, IP - Reported by the developer, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1 - 1.0 µg antibody per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | The optimal concentration should be determined by titration for each individual assay of interest. Clone FF26A/F9 is only reactive to human GAPDH, and not cross-reactive to mouse or rat GAPDH. |
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| Application References(PubMed link indicates BioLegend citation) |
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| Maestre L, et al. 2009. Haematologica. 94:419. Zou L, et al. 2012. J Biol. Chem. 287:7190. PubMed Chen CY, et al. 2012. PNAS. 110:630 PubMed Trifari S, et al. 2013. PNAS. PubMed Liu CC, et al. 2013. Mol. Cell Biol. 33:4334. PubMed Trifari S, et al. 2013. PNAS. 110:18608. PubMed Butin-Israeli V, et al. 2015. Mol. Cell Biol. 35:884. PubMed |
| Product Citations | Trifari S, et al. 2013. Proc Natl Acad Sci U S A. 110:18608. PubMed Strine MS, et al. 2023. PLoS Biol. 21:e3002097. PubMed Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed Wahlig S, et al. 2020. Sci Rep. 1.584027778. PubMed Ong SM, et al. 2018. Cell Death Dis. 9:266. PubMed Zeng L, et al. 2021. J Cell Biol. 220:. PubMed Butin-Israeli V, et al. 2015. Mol Cell Biol. 35:884. PubMed Ruehle S et al. 2017. Journal of neurochemistry. 142(4):521-533 . PubMed Zou L, et al. 2012. J Biol Chem. 287:7190. PubMed Liu R, et al. 2021. Oncol Lett. 21:06. PubMed Liu C, et al. 2013. Mol Cell Biol. 33:4334. PubMed Betriu N, et al. 2021. Cancers (Basel). 13:. PubMed Gorji-Bahri G, et al. 2021. J Cell Biochem. 122:425. PubMed Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed Barbosa JAF, et al. 2021. J Virol. 95:e0097120. PubMed Chen C, et al. 2013. Proc Natl Acad Sci U S A. 110:630. PubMed Zhao J et al. 2019. Transl Psychiatry. 9(1):247 . PubMed Dragoj M, et al. 2017. Invest New Drugs. 10.1007/s10637-017-0494-4. PubMed |
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| RRID | AB_10613283 (BioLegend Cat. No. 649201) AB_10612752 (BioLegend Cat. No. 649202) |
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| Structure | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains. |
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| Distribution | Ubiquitously expressed, locate in cytoplasm and perinuclear region. |
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| Function | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway. |
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| Interaction | Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2 |
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| Biology Area | Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction |
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| Antigen References | 1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. 2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460. 3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527. 4. Zheng L, et al. 2003. Cell. 114:255. 5. Wang Q, et al. 2005. FASEB J. 19:869. 6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA. 103:3405. |
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| Gene ID | 5590 |
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| UniProt | View information about GAPDH on UniProt.org |
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| Clone | FF26A/F9 |
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| Regulatory Status | RUO |
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| Other Names | glyceraldehyde-3-phosphate dehydrogenase |
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| Isotype | Mouse IgG1, κ |
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