Biolegend, 650805, Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10) Antibody
25tests
Catalog Number: 650805
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Modified synthetic peptide conjugated to KLH |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting. |
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| Product Citations | Geddes-McAlister J, et al. 2019. Curr Protoc Immunol. e87:126. PubMed Pai G, et al. 2023. Commun Biol. 6:37. PubMed Benedict B, et al. 2020. Developmental Cell. 52(6):683-698. PubMed de Jong MRW, et al. 2020. Ther Adv Hematol. 11:2040620719898373. PubMed |
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| RRID | AB_2564361 (BioLegend Cat. No. 650805) AB_2564362 (BioLegend Cat. No. 650806) |
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| Structure | H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins. |
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| Distribution | Nucleus |
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| Function | H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability. |
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| Interaction | Two molecules of H3 form a heterotetramer with two molecules of H4. |
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| Biology Area | Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors |
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| Molecular Family | Phospho-Proteins |
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| Antigen References | 1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Hans F, et al. 2001. Oncogene 20:3021. |
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| Regulation | H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination. |
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| Gene ID | 8290 |
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| UniProt | View information about Histone on UniProt.org |
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| Clone | 11D8 |
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| Regulatory Status | RUO |
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| Other Names | Histone-H3, H3 |
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| Isotype | Mouse IgG2b, κ |
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