Biolegend, 651005, Alexa Fluor® 488 anti-STAT3 Phospho (Tyr705) Antibody
25tests
Catalog Number: 651005
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | KLH conjugated modified synthetic peptide |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting. |
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| Product Citations | Jin Y, et al. 2022. EMBO Rep. 23:e55450. PubMed Zhou WJ, et al. 2022. Sci Adv. 8:eabj2488. PubMed Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed Wilmes S, et al. 2021. Elife. 10:. PubMed Gorby C, et al. 2020. Sci Signal. :13. PubMed |
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| RRID | AB_2572083 (BioLegend Cat. No. 651005) AB_2572084 (BioLegend Cat. No. 651006) |
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| Structure | STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain. |
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| Distribution | Ubiquitous. |
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| Function | STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. |
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| Cell Sources | Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation. |
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| Cell Type | Embryonic Stem Cells, Neural Stem Cells |
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| Biology Area | Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors |
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| Molecular Family | Phospho-Proteins |
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| Antigen References | 1. Akira S, et al. 1994. Cell 77:63. 2. Zhang X, et al. 1995. Science 267:1990. 3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30. 4. Simon A, et al. 2000. Science 290:144. 5. Hoey T, et al. 1999. Adv. Immunol. 71:145. |
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| Regulation | The small GTPase Rac1 binds and regulates activity of STAT3. |
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| Gene ID | 6774 |
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| UniProt | View information about STAT3 Phospho Tyr705 on UniProt.org |
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| Clone | 13A3-1 |
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| Regulatory Status | RUO |
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| Other Names | Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES |
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| Isotype | Mouse IgG1, κ |
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