Biolegend, 652418, Alexa Fluor® 488 anti-mouse Ki-67 Antibody
100microg
Catalog Number: 652418
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Category
| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by our Ki-67 protocol below. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed |
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| Product Citations | VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Slusarczyk P, et al. 2023. Elife. 12: . PubMed Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed Sladky VC, et al. 2020. EMBO Rep. :e50893. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed Sen B, et al. 2020. J Bone Miner Res. 35:1149. PubMed Fang Y, et al. 2020. Aging Cell. 19:e13232. PubMed McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed Song W, et al. 2022. Immunity. 55:290. PubMed |
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| RRID | AB_2564236 (BioLegend Cat. No. 652417) AB_2564269 (BioLegend Cat. No. 652418) |
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| Structure | 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats |
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| Distribution | Nucleus and chromosome |
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| Function | Required for cell cycle progression and proliferation |
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| Interaction | Interacts with KIF15; binds to MKI67IP through FHA domain |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143. 2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. 3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. 4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. 5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. 6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294. |
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| Gene ID | 17345 |
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| UniProt | View information about Ki-67 on UniProt.org |
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| Clone | 16A8 |
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| Regulatory Status | RUO |
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| Other Names | KIA, proliferation-related Ki-67 antigen |
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| Isotype | Rat IgG2a, κ |
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