Biolegend, 652509, Alexa Fluor® 488 anti-His Tag Antibody
25microg
Catalog Number: 652509
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Category
| Verified Reactivity | Epitope tag |
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| Reported Reactivity | Species independent |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | KLH-conjugated His-tag peptide |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml (Dilution 1:1000-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Blue Laser (488 nm) |
| Application References(PubMed link indicates BioLegend citation) | Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429. |
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| RRID | AB_2716151 (BioLegend Cat. No. 652509) |
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| Function | Recombinant poly-histidine tag detection |
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| Biology Area | Cell Biology |
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| Gene ID | NA |
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| UniProt | View information about His Tag on UniProt.org |
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| Clone | J099B12 |
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| Regulatory Status | RUO |
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| Other Names | Histidine Tag |
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| Isotype | Mouse IgG1, κ |
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