Biolegend, 653705, Alexa Fluor® 488 anti-Oct4 (Oct3) Antibody
25tests
Catalog Number: 653705
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Partial human Oct4 recombinant protein (1-141aa). |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Blue Laser (488 nm) |
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| Product Citations | Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed Wang W, et al. 2021. Front Pharmacol. 12:707940. PubMed Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 47:101906. PubMed Ababneh NA, et al. 2021. Stem Cell Research. 54:102358. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101925. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 47:101891. PubMed Petters J, et al. 2020. Stem Cell Research. 72.42222222. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed V ölkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed Guzzardo P, et al. 2017. Sci Rep.. 10.1038/s41598-017-16931-z. PubMed |
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| RRID | AB_2562250 (BioLegend Cat. No. 653705) AB_2562251 (BioLegend Cat. No. 653706) |
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| Structure | Belongs to the POU transcription factor family, predicted molecular weight of 39 kD |
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| Distribution | Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells |
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| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. |
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| Interaction | SOX2, SOX8, SOX10, androgen receptor, and ETS2 |
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| Biology Area | Cell Biology, Immunology, Stem Cells, Transcription Factors |
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| Antigen References | 1. Niwa H, et al. 2000. Nature Genet. 24:372. 2. Wernig M, et al. 2007. Nature 448:318. 3. Pesce M, et al. 2001. Stem Cells 19:271. |
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| Gene ID | 5460 |
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| UniProt | View information about Oct4 on UniProt.org |
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| Clone | 3A2A20 |
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| Regulatory Status | RUO |
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| Other Names | POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3 |
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| Isotype | Mouse IgG2b, κ |
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