Biolegend, 656106, Alexa Fluor® 594 anti-SOX2 Antibody
100microg
Catalog Number: 656106
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Full length human SOX recombinant protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Application Notes | This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Product Citations | Castro-Gutierrez R, et al. 2021. Front Endocrinol (Lausanne). 12:707881. PubMed Shin N, et al. 2022. Biotechnol Bioeng. 119:566. PubMed |
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| RRID | AB_2563431 (BioLegend Cat. No. 656106) |
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| Structure | 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding |
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| Distribution | Nucleus |
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| Function | Transcription factor that regulates the expression of the genes involved in embryonic development |
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| Interaction | Interacts with FGFR1, SOX3, and ZSCAN10 |
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| Cell Type | Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors |
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| Antigen References | 1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228. 2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725. 3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772. 4. Jeong CH, et al. 2010. Stem Cells 28:2141. 5. Xiang R, et al. 2011. Br. J. Cancer 104:1410. 6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426. |
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| Gene ID | 6657 |
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| UniProt | View information about SOX2 on UniProt.org |
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| Clone | 14A6A34 |
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| Regulatory Status | RUO |
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| Other Names | SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3 |
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| Isotype | Mouse IgG1, κ |
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