Biolegend, 656109, Alexa Fluor® 488 anti-SOX2 Antibody
25microg
Catalog Number: 656109
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Full length human SOX recombinant protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality testedICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
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| Product Citations | Ceholski D, et al. 2017. Stem Cell Res. 10.1016/j.scr.2017.06.015. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Chen G, et al. 2020. Stem Cell Research. 72.58402778. PubMed Alexandrova S, et al. 2016. Development. 143: 24 - 34. PubMed Coccini T, et al. 2022. Curr Protoc. 2:e423. PubMed |
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| RRID | AB_2563956 (BioLegend Cat. No. 656109) AB_2563957 (BioLegend Cat. No. 656110) |
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| Structure | 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding |
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| Distribution | Nucleus |
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| Function | Transcription factor that regulates the expression of the genes involved in embryonic development |
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| Interaction | Interacts with FGFR1, SOX3, and ZSCAN10 |
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| Cell Type | Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors |
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| Antigen References | 1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228. 2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725. 3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772. 4. Jeong CH, et al. 2010. Stem Cells 28:2141. 5. Xiang R, et al. 2011. Br. J. Cancer 104:1410. 6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426. |
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| Gene ID | 6657 |
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| UniProt | View information about SOX2 on UniProt.org |
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| Clone | 14A6A34 |
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| Regulatory Status | RUO |
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| Other Names | SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3 |
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| Isotype | Mouse IgG1, κ |
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