Biolegend, 656812, Alexa Fluor® 488 anti-Nestin Antibody
100microg
Catalog Number: 656812
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested IHC-P - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 20 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser |
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| Blue Laser (488 nm) |
| Application Notes | For immunohistochemistry use on formalin-fixed paraffin-embedded tissue (IHC-P), antigen retrieval with Citrate Buffer, 10X (Cat. No. 420902) is recommended. |
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| Application References(PubMed link indicates BioLegend citation) | Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC) |
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| Product Citations | Schober S, et al. 2023. Cancer Immunol Immunother. 3621:72. PubMed |
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| RRID | AB_2629793 (BioLegend Cat. No. 656812) |
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| Structure | 1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD. |
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| Distribution | Cytosol |
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| Function | Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation |
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| Interaction | Vimentin, alpha-internexin and FHOD3 |
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| Cell Type | Neural Stem Cells |
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| Biology Area | Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | Intermediate Filaments |
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| Antigen References | Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59. Krupkova O Jr, et al. 2010. Neoplasma. 57:291. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165. Pilkington GJ, et al. 2005. Cell Prolif. 38:423. |
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| Gene ID | 10763 |
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| UniProt | View information about Nestin on UniProt.org |
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| Clone | 10C2 |
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| Regulatory Status | RUO |
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| Other Names | NES |
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| Isotype | Mouse IgG1, κ |
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