Biolegend, 657403, Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3) Antibody
25microg
Catalog Number: 657403
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| Verified Reactivity | Mouse, Rat, Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested IHC-F, 3D IHC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 1.25 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2. |
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| Application References(PubMed link indicates BioLegend citation) | Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Metzner K, et al. 2022. Front Cell Dev Biol. 10:965382. PubMed Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Zhang J, et al. 2020. J Cell Mol Med. 24:4547. PubMed |
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| RRID | AB_2562669 (BioLegend Cat. No. 657403) AB_2562670 (BioLegend Cat. No. 657404) |
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| Structure | 450 amino acids with predicted molecular weight of 50 kD |
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| Distribution | Cytosol |
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| Function | Plays important roles in neuronal cell proliferation and differentiation |
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| Interaction | Alpha tubulin, kinesin and dynein |
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| Cell Type | Mature Neurons |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Microtubules |
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| Antigen References | 1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851. 2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015. 3. Locher H, et al. 2013. Differentiation. 85:173. 4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461. 5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726. 6. Koh Y, et al. 2009. Ann. Oncol. 20:1414. |
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| Gene ID | 10381 |
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| UniProt | View information about Tubulin beta-3 on UniProt.org |
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| Clone | AA10 |
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| Regulatory Status | RUO |
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| Other Names | β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub |
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| Isotype | Mouse IgG2a, κ |
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