Biolegend, 657808, Alexa Fluor® 488 anti-Lin28A Antibody
100tests
Catalog Number: 657808
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human Lin28A from E. coli |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | This clone is specific for Lin28A does not recognize Lin28B. |
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| RRID | AB_2924623 (BioLegend Cat. No. 657807) AB_2924623 (BioLegend Cat. No. 657808) |
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| Structure | 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2) |
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| Distribution | Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules |
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| Function | Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA |
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| Interaction | EIF3S2, NCL and ZCCHC11/TUT4 |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells |
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| Antigen References | Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395-406. Thornton JE, et al. 2012. Trends Cell Biol. 22:474-82. Huang Y. 2012. Wiley Interdiscip Rev RNA. 3:483-94. Cho J, et al. 2012. Cell. 151:765-777. Li N, et al. 2012. J Biol Chem. 287:17386-17397. Zhu H, et al. 2011. Cell 147:81-94. |
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| Gene ID | 79727 |
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| UniProt | View information about Lin28A on UniProt.org |
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| Clone | 14E6-4E6 |
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| Regulatory Status | RUO |
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| Other Names | Protein Lin-28 Homolog A, Lin-28A, CSDD1, ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1 |
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| Isotype | Mouse IgG2a, κ |
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