Biolegend, 658704, Alexa Fluor® 488 anti-Bcl-2 Antibody
100tests
Catalog Number: 658704
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC) Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Tsuzuki H, et al. 2022. Oncotarget. 13:1359. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed |
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| RRID | AB_2563151 (BioLegend Cat. No. 658703) AB_2563152 (BioLegend Cat. No. 658704) |
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| Structure | Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains. |
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| Distribution | Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane. |
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| Function | Regulates apoptosis through controlling mitochondrial fusion and fission. |
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| Interaction | BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP. |
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| Cell Type | B cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation |
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| Antigen References | 1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243. 2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7. 3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414. 4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571. 5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852. |
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| Gene ID | 596 |
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| UniProt | View information about Bcl-2 on UniProt.org |
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| Clone | 100 |
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| Regulatory Status | RUO |
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| Other Names | B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50 |
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| Isotype | Mouse IgG1 |
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