Biolegend, 658705, Alexa Fluor® 647 anti-Bcl-2 Antibody
25tests
Catalog Number: 658705
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC) Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Cabello CM, et al. 2009. Free Radic Biol Med. 220:46. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed Cai J, et al. 2021. eLife. 10:00. PubMed |
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| RRID | AB_2563279 (BioLegend Cat. No. 658705) AB_2563280 (BioLegend Cat. No. 658706) |
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| Structure | Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains. |
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| Distribution | Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane. |
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| Function | Regulates apoptosis through controlling mitochondrial fusion and fission. |
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| Interaction | BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP. |
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| Cell Type | B cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation |
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| Antigen References | 1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243. 2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7. 3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414. 4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571. 5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852. |
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| Gene ID | 596 |
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| UniProt | View information about Bcl-2 on UniProt.org |
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| Clone | 100 |
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| Regulatory Status | RUO |
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| Other Names | B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50 |
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| Isotype | Mouse IgG1 |
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