Biolegend, 663903, Anti-
100microl
Catalog Number: 663903
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| Verified Reactivity | E. coli |
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| Reported Reactivity | Other species |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified recombinant E. coli RNA polymerase β subunit. |
| Preparation | Dried ascites |
| Storage & Handling | Store lyophilized antibody at -20°C. Avoid repeat freeze/thaw cycles. |
| Application | WB - Quality tested ELISA - Verified IP - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconsitution. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3. |
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| Application References(PubMed link indicates BioLegend citation) |
|---|
| Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB) |
| Product Citations | Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed Heinrich K, et al. 2019. MBio. 10. PubMed Ju X, et al. 2019. Nat Microbiol. 1.490972222. PubMed Broder U, Jaeger T, Jenal U 2016. Nat Microbiol. 2:16184. PubMed Ting SY, et al. 2020. Cell Host Microbe. 28(2):313-321.e6. PubMed Ting SY, et al. 2022. Elife. 11:. PubMed Stringer AM, et al. 2020. Elife. 9:00. PubMed Icke C, et al. 2021. eLife. 10:00. PubMed Kouba T, et al. 2020. Nat Commun. 11:6419. PubMed Osuna BA, et al. 2020. Cell Host Microbe. 28:31. PubMed Pribis JP et al. 2019. Mol Cell. 74(4):785-800 . PubMed |
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| RRID | AB_2564524 (BioLegend Cat. No. 663903) |
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| Structure | 1252 amino acids with a predicted molecular weight of approximately 150 kD. |
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| Distribution | Bacteria. |
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| Function | Transcriptional regulator in bacteria. |
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| Biology Area | Cell Biology, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. |
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| Gene ID | 948488 |
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| UniProt | View information about RNA Polymerase beta on UniProt.org |
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| Clone | 8RB13 |
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| Regulatory Status | RUO |
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| Other Names | RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta |
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| Previously | NeoClone Catalog# W0023 |
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| Isotype | Mouse IgG2b, κ |
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