Biolegend, 673801, Anti-
1ml
Catalog Number: 673801
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| Verified Reactivity | E. coli |
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| Reported Reactivity | Other species |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified recombinant E. coli RNA polymerase β subunit. |
| Formulation | 50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included. |
| Storage & Handling | Store at 4°C until use. Use at room temperature. |
| Application | IP - Quality tested |
| Recommended Usage | Resin binding capacity is approximately 0.15 mg/mL. To elute, use TE, 0.7M ammonium sulfate, 40% propylene glycol. |
| Application Notes | Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3. |
| Application References(PubMed link indicates BioLegend citation) |
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| Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB) |
| RRID | AB_3105970 (BioLegend Cat. No. 673801) |
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| Structure | 1252 amino acids with a predicted molecular weight of approximately 150 kD. |
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| Distribution | Bacteria. |
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| Function | Transcriptional regulator in bacteria. |
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| Biology Area | Cell Biology, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. |
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| Gene ID | 948488 |
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| UniProt | View information about RNA Polymerase beta on UniProt.org |
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| Clone | 8RB13 |
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| Regulatory Status | RUO |
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| Other Names | RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta |
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| Previously | NeoClone Catalog# S0004 |
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| Isotype | Mouse IgG2b, κ |
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