Biolegend, 674010, Alexa Fluor® 647 anti-Nanog Antibody
100microg
Catalog Number: 674010
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Full length human recombinant protein expressed in bacteria |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
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| Product Citations | Chen Z, et al. 2024. J Extracell Vesicles. e12409:13. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 72.77986111. PubMed Ibuki M, et al. 2019. Regen Ther. 0.551388889. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed |
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| RRID | AB_2632605 (BioLegend Cat. No. 674010) |
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| Structure | 305 amino acids with a predicted molecular weight of approximately 35 kD. |
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| Distribution | Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation. |
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| Function | Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. |
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| Interaction | Interacts with SMAD1, SALL4, and ZNF281/ZFP281. |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors |
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| Antigen References | 1. Hwang CC, et al. 2014. PLoS One 9:e105676. 2. Torres-Padilla ME, et al. 2014. Development 141:2173. 3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741. 4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207. 5. Kim J, et al. 2008. Cell 132:1049. 6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081. 7. Yu J, et al. 2007. Science 318:1917. |
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| Gene ID | 79923 |
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| UniProt | View information about Nanog on UniProt.org |
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| Clone | 23D2-3C6 |
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| Regulatory Status | RUO |
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| Other Names | Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48 |
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| Isotype | Mouse IgG1, κ |
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