Biolegend, 674206, Alexa Fluor® 488 anti-Nanog Antibody
100microg
Catalog Number: 674206
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Full length NANOG recombinant protein expressed in E. coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
| Product Citations | Wang G, et al. 2016. Cell Biosci. 41:6. PubMed |
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| RRID | AB_2566687 (BioLegend Cat. No. 674206) |
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| Structure | 305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding. |
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| Interaction | Interacts with ZFP281, SMAD1, and SALL4. |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Cell Biology, Immunology, Stem Cells, Transcription Factors |
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| Molecular Family | Nuclear Markers |
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| Antigen References | 1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587. 2. Villasante A, et al. 2011. Cell Cycle 10:1488. 3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222. 4. Pan G, et al. 2007. Cell Res. 17:42. 5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093. 6. Silva J, et al. 2009. Cell 138:722. |
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| Gene ID | 79923 |
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| UniProt | View information about Nanog on UniProt.org |
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| Clone | 16H3A48 |
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| Regulatory Status | RUO |
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| Other Names | Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48 |
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| Isotype | Mouse IgG1, κ |
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