Biolegend, 686409, Alexa Fluor® 488 anti-STAT1 Phospho (Ser727) Antibody
25tests
Catalog Number: 686409
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human STAT1 peptide phosphorylated at Ser 727. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Product Citations | Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed |
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| RRID | AB_2650783 (BioLegend Cat. No. 686409) AB_2650784 (BioLegend Cat. No. 686410) |
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| Structure | 750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization. |
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| Distribution | Translocates to the nucleus when phosphorylated. |
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| Function | Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6. |
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| Interaction | Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src. |
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| Biology Area | Cell Biology, Signal Transduction |
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| Molecular Family | Nuclear Markers, Phospho-Proteins |
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| Antigen References | 1. Durbin JE, et al. 1996. Cell. 84:443. 2. Darnell JE Jr, et al. 1994. Science 264:1415. 3. Chen X, et al. 1998. Cell. 93:827. 4. Ramana CV, et al. 2000. Oncogene. 19:2619. |
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| Gene ID | 6772 |
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| UniProt | View information about STAT1 Phospho Ser727 on UniProt.org |
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| Clone | A15158B |
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| Regulatory Status | RUO |
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| Other Names | Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84 |
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| Isotype | Mouse IgG1, κ |
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