Biolegend, 687603, Alexa Fluor® 647 anti-Histone H3.1 Phospho (Ser28) Antibody
25tests
Catalog Number: 687603
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Histone H3.1 S28ph peptide (21-39)(ATKAARK(phS)APATGGVKKPH), Freund's complete adjuvant. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| RRID | AB_2616946 (BioLegend Cat. No. 687603) AB_2616947 (BioLegend Cat. No. 687604) |
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| Structure | H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins. |
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| Distribution | Nucleus. |
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| Function | H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Therefore, histones limit the accessibility of DNA by providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. |
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| Interaction | Two molecules of H3 form a heterotetramer with two molecules of H4. |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability |
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| Molecular Family | Phospho-Proteins |
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| Antigen References | 1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Yoshimi T, et al. 2013. Monoclon. Immunodiagn. Immunother. 32:119. |
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| Gene ID | 8350 |
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| UniProt | View information about Histone H3.1 on UniProt.org |
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| Clone | 5D10D4 |
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| Regulatory Status | RUO |
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| Other Names | Histone-H3 |
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| Isotype | Rat IgG2a, κ |
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