Biolegend, 698405, Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac) Antibody
25microg
Catalog Number: 698405
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| Verified Reactivity | Human, Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Histone H3 K9ac peptide |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
| Application Notes | This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). |
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| RRID |
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| AB_2832850 (BioLegend Cat. No. 698405) AB_2832851 (BioLegend Cat. No. 698406) |
| Structure | Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational. |
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| Distribution | Nucleus |
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| Function | Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones. |
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| Interaction | Histones 2A, 2B, 4, HDAC |
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| Biology Area | Cell Biology, Chromatin Remodeling/Epigenetics |
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| Antigen References | 1. Zhang, et al. 2017. Nat. Commun. 8: 14799. 2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394. 3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333. 4. Li B, et al. 2016. Sci. Rep. 6: 26542. 5. Li D, et al. 2014, Mol. Cancer. 13:26. |
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| Gene ID | 8350 |
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| UniProt | View information about Histone H3 on UniProt.org |
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| Clone | 2G1F9 |
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| Regulatory Status | RUO |
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| Isotype | Rat IgG2a, κ |
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