Biolegend, 699303, Alexa Fluor® 594 anti-Vimentin Antibody
25microg
Catalog Number: 699303
Send an RFQ.
Category
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Application Notes | This antibody does not cross-react with human (in-house tested). May be weakly reactive with human Vimentin by WB. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| RRID | AB_2728517 (BioLegend Cat. No. 699303) AB_2728518 (BioLegend Cat. No. 699304) |
|---|
| Structure | 466 amino acids with a predicted molecular weight of 54 kD. |
|---|
| Distribution | Cytoplasm. |
|---|
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
|---|
| Interaction | HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3. |
|---|
| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
|---|
| Molecular Family | Intermediate Filaments |
|---|
| Antigen References | 1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1. 2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345. 3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429. 4. Scanlon CS, et al. 2013. J Dent Res. 92:114. 5. Thebault S, et al. 2002. J Immunol. 169:4046. 6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142. 7. Rose ML. 2013. Hum Immunol. 74:1459. |
|---|
| Gene ID | 22352 |
|---|
| UniProt | View information about Vimentin on UniProt.org |
|---|
| Clone | W16220A |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113 |
|---|
| Isotype | Rat IgG2a, κ |
|---|
