Biolegend, 699305, Alexa Fluor® 488 anti-Vimentin Antibody
25microg
Catalog Number: 699305
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested ICFC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | This antibody does not cross-react with human (in-house tested). May be weakly reactive with human Vimentin by WB. |
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| RRID | AB_2888889 (BioLegend Cat. No. 699305) AB_2888889 (BioLegend Cat. No. 699306) |
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| Structure | 466 amino acids with a predicted molecular weight of 54 kD. |
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| Distribution | Cytoplasm. |
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| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
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| Interaction | HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3. |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Antigen References | 1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1. 2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345. 3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429. 4. Scanlon CS, et al. 2013. J Dent Res. 92:114. 5. Thebault S, et al. 2002. J Immunol. 169:4046. 6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142. 7. Rose ML. 2013. Hum Immunol. 74:1459. |
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| Gene ID | 22352 |
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| UniProt | View information about Vimentin on UniProt.org |
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| Clone | W16220A |
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| Regulatory Status | RUO |
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| Other Names | Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113 |
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| Isotype | Rat IgG2a, κ |
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