Biolegend, 699307, Alexa Fluor® 647 anti-Vimentin Antibody
25microg
Catalog Number: 699307
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested ICFC - Verified SB - Community verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | This antibody does not cross-react with human (in-house tested). May be weakly reactive with human Vimentin by WB. |
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| Additional Product Notes | This product has been verified for IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page. |
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| RRID | AB_2888890 (BioLegend Cat. No. 699307) AB_2888890 (BioLegend Cat. No. 699308) |
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| Structure | 466 amino acids with a predicted molecular weight of 54 kD. |
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| Distribution | Cytoplasm. |
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| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
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| Interaction | HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3. |
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| Biology Area | Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Intermediate Filaments |
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| Antigen References | 1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1. 2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345. 3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429. 4. Scanlon CS, et al. 2013. J Dent Res. 92:114. 5. Thebault S, et al. 2002. J Immunol. 169:4046. 6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142. 7. Rose ML. 2013. Hum Immunol. 74:1459. |
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| Gene ID | 22352 |
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| UniProt | View information about Vimentin on UniProt.org |
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| Clone | W16220A |
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| Regulatory Status | RUO |
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| Other Names | Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113 |
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| Isotype | Rat IgG2a, κ |
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