Biolegend, 712054, Biotinylated Recombinant Human Cadherin-17 (carrier-free)
25microg
Catalog Number: 712054
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| Source | Recombinant human Cadherin-17, amino acid Gln23-Met787 (Accession No. Q12864) was expressed in 293E cells. The C-terminal includes Avi-6His. |
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| Molecular Mass | The 801 amino acid recombinant protein has a predicted molecular mass of approximately 91 kD. The DTT-reduced and non-reduced proteins migrates at approximately 80 - 115 kD by SDS-PAGE. The predicted N-terminall amino acid is Gln. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg protein as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Biotinylated recombinant human CDH17 binds to immobilized recombinant human Galectin-9 in a dose-dependent manner. The ED50 for this effect is 1 - 6 µg/mL |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer |
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| Distribution | Intestinal cells, B cells in embryonic development and fetal liver, memory B cells, cancer cells, epithelial cells in small intestine, colon, and pancreatic ducts |
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| Function | Cell- cell adhesion, tissue morphogenesis, signal transduction |
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| Interaction | CDH1, p120-catenin |
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| Ligand/Receptor | α2β1 integrin |
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| Bioactivity | Measured by its ability to bind biotinylated recombinant human Galectin-9 |
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| Cell Type | Embryonic Stem Cells, Epithelial cells, Erythrocytes |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology |
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| Molecular Family | Adaptor Proteins |
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| Antigen References | Dantzig AH, et al. 1994. Science. 264:430-3. Liu LX. et al. 2009. Hepatology. 50:1453-63. Ding ZB, et al. 2009. Cancer. 115:4753-65. Bartolmäs T, et al. 2012. Cell Mol Life Sci. 69:3851-62. Feng Z. et al. 2022. Nat Cancer. 3:581. Liu X. et al. 2019. Cancer Lett. 454:204-214. Funakoshi S, et al. 2015. PLoS One.10:e0117566. |
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| Gene ID | 1015 |
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| UniProt | View information about Cadherin-17 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | LI cadherin (liver-intestine), Cadherin 17, Cadherin17,Cadherin-17, CDH16, CDH17, FLJ26931, HPT-1 cadherin, HPT1,HPT-1, human intestinal peptide-associated transporter HPT-1 |
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