Biolegend, 713155, Biotinylated Recombinant Human HVEM-Fc Chimera (carrier-free)
50microg
Catalog Number: 713155
Send an RFQ.
Category
Loading…
| Source | Biotinylated recombinant human HVEM, amino acid Leu39-Val202 (Accession No. Q92956) with a C-terminal Xa-hFc-Avi tag was expressed in CHO cells. |
|---|---|
| Molecular Mass | The 416 amino acid recombinant protein has a predicted molecular mass of approximately 45.8 kD. The DTT-reduced and non-reduced proteins migrate at approximately 62 kD and 80 - 115 kD respectively by SDS-PAGE. The N-terminal amino acid is Leu. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilized from 0.22 µm filtered protein solution is in PBS, 5% trehalose, pH7.2 |
| Endotoxin Level | Less than 0.1 EU per µg protein as determined by the LAL method. |
| Concentration | 50 µg size, lyophilized |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Storage & Handling |
|---|
| Unopened vial can be stored between 2°C and 4°C for up to two weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile PBS, pH 7.2. Before reconstitution, make sure the sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months. |
| Activity | Biotinylated recombinant human HVEM-Fc Chimera binds to immobilized recombinant human BTLA (Cat. No. 775704) in a dose-dependent manner. The ED50 for this effect is 2 – 20 ng/mL |
|---|
| Application | Bioassay |
|---|
| Application Notes | Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
|---|
| Structure | Dimer |
|---|
| Distribution | Spleen, thymus, lungs, macrophages, dendritic cells, monocytes, neutrophils, B cells and T cells |
|---|
| Function | HVEM plays a role in mucosal host defense, autoimmune and inflammatory diseases, and tumor progression |
|---|
| Interaction | T cells, B cells, macrophages, dendritic cells, all intraepithelial lymphocytes, and epithelial cells, and natural killer cells |
|---|
| Ligand/Receptor | BTLA, CD160, LIGHT, lymphotoxin-α |
|---|
| Bioactivity | Measured by its ability to bind human BTLA |
|---|
| Cell Type | Hematopoietic stem and progenitors, Mesenchymal Stem Cells |
|---|
| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells |
|---|
| Molecular Family | CD Molecules |
|---|
| Antigen References | Pasero C. et al. 2012. Curr. Opin. Pharmacol. 12:478. Hsu H. et al. 1997. J. Biol. Chem. 272:13471. Mauri DN. et al. 1998. Immunity. 8:21. Montgomery RI. et al. 1996. Cell. 87:427. Shui JW. et al. 2012. Nature. 488:222. |
|---|
| Gene ID | 8764 |
|---|
| UniProt | View information about HVEM/TNFRSF14 on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Herpiesvirus Entry Mediator, HVEA, TR2, Tumor Necrosis Factor Receptor Superfamily 14, TNFRSF14 |
|---|
