Biolegend, 715854, Biotinylated Recombinant Human SLAMF4 (CD244/2B4)-Fc Chimera (carrier-free)
25microg
Catalog Number: 715854
Send an RFQ.
Category
Loading…
| Source | Human SLAMF4 (CD244/2B4), amino acid (Cys22-Arg221) (Accession# NP_057466.1), with a linker, a C-terminal Human IgG (Pro100-Lys330), and an Avi-tag, was expressed in CHO cells. The Recombinant Human CD244-Avi tag was site-specifically biotinylated by the enzyme BirA. |
|---|---|
| Molecular Mass | The 450 amino acid recombinant protein has a predicted molecular mass of approximately 50.4 kD. The DTT-reduced and non-reduced proteins migrate at approximately 65 -85 kD and 110 – 145 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Cys. |
| Purity | > 95%, as determined by Coomassie-stained SDS-PAGE |
| Formulation | 0.22 µm filtered protein solution is in PBS, 5% Sucrose, pH 7.4 |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Storage & Handling |
|---|
| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | When Recombinant Human SLAMF2 (Cat. No. 788706) is immobilized at 5 µg/mL, Biotinylated Recombinant Human SLAMF4 (CD244/2B4) binds in a dose-dependent manner with an ED50 range of 15 – 90 ng/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding. |
|---|
| Application | Biossay |
|---|
| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
|---|
| Structure | Dimer |
|---|
| Distribution | NK cells, monocytes and basophils, TCR γ/δ T cells, and a subset of CD8+ T cells |
|---|
| Function | Engagement of SLAMF4 with CD48 stimulates cell-mediated cytotoxicity and cytokine secretion |
|---|
| Interaction | NK cells, subsets of T cells |
|---|
| Ligand/Receptor | SLAMF2/CD48 |
|---|
| Bioactivity | The bioactivity was measured by immobilizing the Recombinant Human SLAMF2 binding with Biotinylated Recombinant Human SLAMF4 (CD244/2B4) in a dose-dependent manner using the functional ELISA. |
|---|
| Cell Type | Basophils, Monocytes, NK cells, T cells |
|---|
| Biology Area | Immunology |
|---|
| Molecular Family | CD Molecules, Soluble Receptors |
|---|
| Antigen References | Parolini S, et al. 2000. J Exp Med. 192:337-46. Watzl C, et al. 2000. J Immunol. 165:3545-8. Brown MH, et al. 1998. J Exp Med. 188:2083-90. Watzl C, et al. 2000. J Immunol. 165:3545-8. Eissmann P, et al. 2005. Blood. 105:4722-9. Latchman Y, et al. 1998. J Immunol. 161:5809-12. Speiser DE, et al. 2001. J Immunol. 167:6165-70. Lee KM, et al. 2003. J Immunol. 170:4881-5. Kambayashi T, et al. 2001. J Immunol. 167:6706-10. Tangye SG, et al. 1999. J Immunol. 162:6981-5. Nakajima H, et al. 1999. Eur J Immunol. 29:1676-83. Schuhmachers G, et al. 1995. Eur J Immunol. 25:1117-20 Sivori S, et al. 2000. Eur J Immunol. 30:787-93. |
|---|
| Gene ID | 51744 |
|---|
| UniProt | View information about SLAMF4 on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | SLAMF4, SLAM family member 4, 2B4, Natural killer cell receptor2B4, NK cell activation-inducing ligand, NAIL, NK cell type Ireceptor protein 2B4, NKR2B4, h2B4, signaling lymphocyticactivation molecule 4, CD244 |
|---|
