Biolegend, 715856, Biotinylated Recombinant Human SLAMF4 (CD244/2B4)-Fc Chimera (carrier-free)
100microg
Catalog Number: 715856
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| Source | Human SLAMF4 (CD244/2B4), amino acid (Cys22-Arg221) (Accession# NP_057466.1), with a linker, a C-terminal Human IgG (Pro100-Lys330), and an Avi-tag, was expressed in CHO cells. The Recombinant Human CD244-Avi tag was site-specifically biotinylated by the enzyme BirA. |
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| Molecular Mass | The 450 amino acid recombinant protein has a predicted molecular mass of approximately 50.4 kD. The DTT-reduced and non-reduced proteins migrate at approximately 65 -85 kD and 110 – 145 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Cys. |
| Purity | > 95%, as determined by Coomassie-stained SDS-PAGE |
| Formulation | 0.22 µm filtered protein solution is in PBS, 5% Sucrose, pH 7.4 |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | When Recombinant Human SLAMF2 (Cat. No. 788706) is immobilized at 5 µg/mL, Biotinylated Recombinant Human SLAMF4 (CD244/2B4) binds in a dose-dependent manner with an ED50 range of 15 – 90 ng/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding. |
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| Application | Biossay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Dimer |
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| Distribution | NK cells, monocytes and basophils, TCR γ/δ T cells, and a subset of CD8+ T cells |
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| Function | Engagement of SLAMF4 with CD48 stimulates cell-mediated cytotoxicity and cytokine secretion |
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| Interaction | NK cells, subsets of T cells |
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| Ligand/Receptor | SLAMF2/CD48 |
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| Bioactivity | The bioactivity was measured by immobilizing the Recombinant Human SLAMF2 binding with Biotinylated Recombinant Human SLAMF4 (CD244/2B4) in a dose-dependent manner using the functional ELISA. |
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| Cell Type | Basophils, Monocytes, NK cells, T cells |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Soluble Receptors |
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| Antigen References | Parolini S, et al. 2000. J Exp Med. 192:337-46. Watzl C, et al. 2000. J Immunol. 165:3545-8. Brown MH, et al. 1998. J Exp Med. 188:2083-90. Watzl C, et al. 2000. J Immunol. 165:3545-8. Eissmann P, et al. 2005. Blood. 105:4722-9. Latchman Y, et al. 1998. J Immunol. 161:5809-12. Speiser DE, et al. 2001. J Immunol. 167:6165-70. Lee KM, et al. 2003. J Immunol. 170:4881-5. Kambayashi T, et al. 2001. J Immunol. 167:6706-10. Tangye SG, et al. 1999. J Immunol. 162:6981-5. Nakajima H, et al. 1999. Eur J Immunol. 29:1676-83. Schuhmachers G, et al. 1995. Eur J Immunol. 25:1117-20 Sivori S, et al. 2000. Eur J Immunol. 30:787-93. |
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| Gene ID | 51744 |
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| UniProt | View information about SLAMF4 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | SLAMF4, SLAM family member 4, 2B4, Natural killer cell receptor2B4, NK cell activation-inducing ligand, NAIL, NK cell type Ireceptor protein 2B4, NKR2B4, h2B4, signaling lymphocyticactivation molecule 4, CD244 |
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