Biolegend, 717455, Recombinant Human Frizzled-4-Fc Chimera (carrier-free)
50microg
Catalog Number: 717455
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| Source | Recombinant human Frizzled-4, amino acid Phe37-Glu180 (Accession No. Q9ULV1), with a proline at N-terminus and a C-terminal Xa-mutant hFc (C103S), was expressed in CHO cells. |
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| Molecular Mass | The 382 amino acid recombinant protein has a predicted molecular mass of approximately 43.0 kD. The DTT-reduced and non-reduced proteins migrate at approximately 50 - 62 kD and 80 - 115 kD by SDS-PAGE respectively. The N-terminal amino acid is Pro. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per µg protein as determined by the LAL method. |
| Concentration | 50 µg size is bottled at 0.2 mg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | ED50= 0.25 - 2.5 µg/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by recombinant human Wnt3a. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Dimer |
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| Distribution | Kidney, lung, brain, liver, neuronal stem cells, fetal neuronal progenitor cells and neuronal intestinal cells |
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| Function | Receptor for Wnt proteins that function in the canonical Wnt/beta-catenin signaling pathway, embryonic development, tissue/cell polarization and proliferation |
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| Ligand/Receptor | Norrin, Wnt proteins |
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| Bioactivity | Measured by its ability to inhibit recombinant Wnt3a activity in Topflash assay |
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| Biology Area | Angiogenesis, Cell Biology, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Qian Y, et al. 2024. Nat Commun. 15:7644. Kushwaha P, et al. 2020. J Cell Physiol. 235:6673. Caceres L, et al. 2019. Dev Dyn. 248:1243. Neckel PH, et al. 2019. Cells. 8:792. DeBruine ZJ, et al. 2017. Genes Dev. 31:916. Rickman M, et al. 2023. J Cell Sci. 136: jcs260449. Descamps B, et al. 2012. Circ Res. 110:47. Shen G, et al. 2012. Cell Res. 25:1078. |
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| Gene ID | 8322 |
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| UniProt | View information about Frizzled-4 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Frizzled4, Frizzled-4, FZ-4, FZD4, FZD4S, FzE4, hFZ4, CD344, CD344 antigen,Frizzled (Drosophila) homolog 4, EVR1, GPCR, WNT receptor frizzled-4 |
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