Biolegend, 717755, Biotinylated Recombinant Human MUC16 (carrier-free)
50microg
Catalog Number: 717755
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| Source | Human MUC16, amino acids (Met13360 – Gln14347) (Accession# Q8WXI7), was expressed in CHO cells. The carboxy terminus contains 6His-Avi tag. Human MUC16-Avi tag was site-specifically biotinylated by enzyme BirA. |
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| Molecular Mass | The 1014 amino acid recombinant protein has a predicted molecular mass of approximately 112.5 kD. The protein migrates at approximately 140 - 170 kD in DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilized from 0.22 µm filtered protein solution in PBS pH7.2, 5% trehalose. |
| Endotoxin Level | Less than 0.1 EU per µg of cytokine as determined by the LAL method. |
| Concentration | 50 µg size lyophilized |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in PBS pH7.2. Before reconstitution, make sure sterile PBS pH7.2 and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS pH7.2) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months. |
| Activity | Recombinant human MUC16 binds to immobilized recombinant human Mesothelin-Fc chimera (Cat. No. 593202) in a dose-dependent manner. The ED50 for this effect is 0.07 – 0.42 ng/mL. |
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| Application | Bioassay |
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| Application Notes | Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer |
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| Distribution | Epithelial cells, limbal epithelium (corneal epithelial cells), endometrial epithelial cells. Multiple cancers, epithelial ovarian cancer, pancreatic cancer, endometrial carcinoma, esophageal, gastric, and colorectal adenocarcinomas. Interstitial lung disease. |
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| Function | Maintain mucosal integrity and provide lubrication, protective barrier against pathogens in the limbal epithelium. |
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| Interaction | Mesothelial cells, ovarian tumors, mesotheliomas |
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| Ligand/Receptor | Mesothelin, Galectin 1 |
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| Bioactivity | MUC16 binds to immobilized mesothelin in a dose dependent manner. |
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| Cell Type | Endothelial cells, Epithelial cells |
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| Biology Area | Cancer Biomarkers, Immuno-Oncology, Immunology |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | Yin BW and Lloyd KO. 2001. J Biol Chem. 276:27371. Streppel MM, et al. 2012. Hum Pathol. 43:1755. Gubbels JAA, et al, 2006. Mol Cancer. 5:50. Seelenmeyer C, et al, 2003. J Cell Sci. 116:1305. Wang F, et al, 2021. J Cell Mol Med. 25:3031. Zhang XJ, et al, 2024. Clin Exp Med 24:101. |
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| Gene ID | 94025 |
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| UniProt | View information about MUC16 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CA125, CA-125, MUC-16, CA125 ovarian cancer antigen, cancerantigen 125 |
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