Biolegend, 718858, Biotinylated Recombinant Human TNFSF18 (GITRL)-Fc Chimera (carrier-free)
50microg
Catalog Number: 718858
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| Source | Recombinant human TNFSF18, amino acid Gln50-Ser177 (Accession No. Q9UNG2.3) with a N-terminal Avi- Fc tag was expressed in CHO cells. |
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| Molecular Mass | The 391 amino acid recombinant protein has a predicted molecular mass of approximately 43.3 kD. The DTT-reduced and non-reduced proteins migrate at approximately 50 - 62 kD and 80 - 100 kD respectively by SDS-PAGE. The N-terminal amino acid is Gly. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Lyophilized from 0.22 µm filtered protein solution is in PBS, 5% trehalose, pH7.2 |
| Endotoxin Level | Less than 0.1 EU per µg protein as determined by the LAL method. |
| Concentration | 50 µg size, Lyophilized |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 4°C for up to two weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile PBS, pH 7.2. Before reconstitution, make sure the sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months. |
| Activity | Human TNFSF18 (GITRL) binds to immobilized recombinant human TNFRSF18 (GITR) (Cat. No. 764304) in a dose-dependent manner. The ED50 for this effect is 0.6 – 3 ng/mL. |
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| Application | Bioassay |
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| Application Notes | Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Dimer |
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| Distribution | Macrophages, B cells, dendritic cells, and endothelial cells |
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| Function | TNFSF18 which induced by inflammatory processes plays roles in regulating T cell activation and natural killer cell anti-tumor activity |
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| Interaction | CD4+CD25+ regulatory T cells (Treg), subsets of thymocytes, lymph node cells, and splenocytes |
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| Ligand/Receptor | GITR/TNFRSF18 |
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| Bioactivity | Measured by its ability to bind human TNFRSF18 (GITR) |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Cell Proliferation and Viability, Stem Cells |
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| Molecular Family | Cytokines/Chemokines, Growth Factors |
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| Antigen References | Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433. Kim JD, et al. 2003. Genes Immun. 4:564. Azuma M. 2010. Crit. Rev. Immunol. 30:547. Hanabuchi S, et al. 2006. Blood. 107:3617. Muriglan SJ, et al. 2004. J. Exp. Med. 200:149. Shevach EM and Stephens GL. 2006. Nat. Rev. Immunol. 6:613. Baltz K, et al. 2007. FASEB J. 21:2442. Placke T, et al. 2010. Clin. Dev. Immunol. 2010:239083. |
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| Gene ID | 8995 |
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| UniProt | View information about TNFSF18 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | TL6, AITRL, GITRL, GITR Ligand, Tumor necrosis factor superfamily,Member 18 |
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