Biolegend, 740610, LEGENDplex™ Human Hematopoietic Stem Cell Panel (12-plex) with Filter Plate

Hematopoietic stem cells (HSCs) are located in the red bone marrow, and differentiate to give rise to myeloid, erythroid, and lymphoid lineages of cells which can further differentiate into specialized blood cells. The differentiation of HSCs into specialized cells is orchestrated by a complex interplay between cytokines, growth factors, and chemokines which are considered important lineage-specific markers. The LEGENDplex™ Human Hematopoietic Stem Cell Panel (12-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 12 key targets involved in hematopoietic stem cell differentiation and lineage specific markers including IL-6, FLT3L, GM-CSF, IL-3, IL-34, IL-11, SCF, LIF, CXCL12 (SDF-1), IL-15, M-CSF, and IL-7. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples. The LEGENDplex™ Human Hematopoietic Stem Cell Panel is designed to allow flexible customization within the panel. It can also be divided into sub-panels such as: LEGENDplex™ Human HSC Lymphoid Panel (9-plex) LEGENDplex™ Human HSC Erythroid Panel (6-plex) LEGENDplex™ Human HSC Myeloid Panel (7-plex) Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.