Biolegend, 740866, LEGENDplex™ HU Immune Checkpoint Panel 1 - TC (12-plex) w/FP

The LEGENDplex™ HU Immune Checkpoint Panel 1 (12-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 12 key immune checkpoint proteins: sCD25 (IL-2Ra), 4-1BB, sCD27, B7.2 (CD86), Free Active TGF-β1, CTLA-4, PD-L1, PD-L2, PD-1, Tim-3, LAG-3, and Galectin-9. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples. The LEGENDplex™ HU Immune Checkpoint Panel 1 is configured as shown below depending on sample types and required dilutions: Catalog No. Plex Size Targets Recommended Sample Type Recommended Dilution Factor 740866 740867 12-plex sCD25 (IL-2Ra), 4-1BB, sCD27, B7.2 (CD86), Free Active TGF-β1, CTLA-4, PD-L1, PD-L2, PD-1, Tim-3, LAG-3, Galectin-9 Tissue Culture Varies 740961 740962 10-plex sCD25 (IL-2Ra), 4-1BB, B7.2 (CD86), Free Active TGF-β1, CTLA-4, PD-L1, PD-1, Tim-3, LAG-3, Galectin-9 Serum, Plasma 2 740963 740964 2-plex sCD27, PD-L2 Serum, Plasma 100 The LEGENDplex™ HU Immune Checkpoint Panel 1 is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel. This assay is for research use only.