Biolegend, 741235, LEGENDplex™ Rat Tfh Panel V02 (5-plex) w/ FP

Upon antigen presentation by APCs (Antigen Presenting Cells), naive CD4 + T cells can differentiate into one of several lineages of T helper (Th) cells, including Th1, Th2, Th17, Th9, Tfh, Th22, and ThGM as defined by their patterns of cytokine production and functions. T helper cells play a critical role in mediating adaptive immunity to a variety of pathogens. They secrete cytokines to stimulate various effector cells, such as cytotoxic T cells, B cells and macrophages. Accurate measurement of Th cytokine expression is critical in understanding the nature of the immune response and many disease processes. The LEGENDplex™ Rat Tfh Panel is a bead-based multiplex assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 5 rat cytokines, including IL-10, IFN-γ, TNF-α, IL-4, and IL-6, which are collectively secreted by Th1, Th2, Th9, Th17, Th22, ThGM, and Tfh cells. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA method. The panel has been validated for use on cell culture supernatant, serum and plasma samples. This assay is for research use only.