Biolegend, 741451, LEGENDplex™ Hu Tumor-Assoc. Macrophage Panel (5-plex) w/ FP

Macrophages are produced by differentiation of monocytes in response to an infection or tissue damage. They play important roles in the immune response and in the maintenance of tissue homeostasis, such as recognizing, engulfing, destroying, and clearing foreign or harmful substances including pathogens, dying or dead cells, cellular debris and tumor cells. Many tumors recruit monocytes from circulation and transform them into an immunosuppressive subset called Tumor-Associated Macrophages (TAMs). TAMs are highly plastic and can alter their phenotype between M1 proinflammatory and M2 anti-inflammatory according to their location and surrounding cytokine milieu in the tumor microenvironment. M1 macrophages are considered to be functioning in the recognition and destruction of cancer cells whereas M2 macrophages are thought to promote proliferation, angiogenesis, and metastasis of cancer cells. Therefore, the phenotypes of TAMs and the soluble factors produced by them influence various aspects of cancer progression and patient prognosis. The LEGENDplex™ Human Tumor-Associated Macrophage Panel is a beadbased multiplex assay, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 5 key targets involved in the interplay of macrophages with the tumor microphage environment, including MMP-9, CCL18, CD163, PDGF-BB, and YKL40. This assay panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.