Biolegend, 741474, LEGENDplex™ Human Allergy/Asthma Panel (2-plex) w/ FP

Allergic diseases including asthma are mediated by T-helper 2 (Th2)-biased immune responses together with isotype switching of B cells to generate IgE antibodies specific for common environmental allergens. Th2 cytokines such as IL-4, IL-5, IL-9 and IL-13 play a significant role in allergic reactions, which can be further amplified by IL-33 with its ST2 binding and activation. TSLP, produced by epithelial cells in the airways, also drive those Th2 cytokines by stimulating dendritic cells into maturation. Periostin, another molecule secreted by airway epithelial cells, can be upregulated by IL-4 and IL-13. Asthma and allergic inflammation are also induced through RAGE receptor binding of advanced glycation end products. Moreover, MMP-9 of the matrix metalloproteinase family is responsible for extracellular matrix degradation and airway remodeling, with its increase correlated with asthma severity. Similarly, YKL-40, a chitinase-like protein, is significantly elevated in asthmatic patients’ serum, indicating a potential biomarker for the disease. CCL11 (Eotaxin), a CC chemokine, is another crucial player in recruitment and migration of eosinophils from peripheral blood into the lung to perpetuate asthma and allergic inflammation through CCR3 binding, which results in subsequent airway remodeling through profibrogenic activity. Examining the various cytokines and molecules associated with allergies and asthma can lead to improved insights for developing effective treatments in the future. The LEGENDplex™ Human Allergy/Asthma Panel is a bead-based multiplex assay, utilizing fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 2 human analytes related to allergy and asthma, including YKL-40 and MMP-9. This panel assay provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant samples.