Biolegend, 741507, LEGENDplex™ Human Phospho-Tau Panel (3-plex) w/ FP

Tau is a 441-amino acid protein that binds along microtubules to stabilize them. The hyperphosphorylation of Tau reduces its affinity for microtubules, leading to destabilization. Tau in healthy neurons carries multiple phosphate groups. Once highly phosphorylated, Tau protein assembles into higher order structures, including oligomers and neurofibrillary tangles (NFT). Elevated phosphorylation and aggregation of Tau are widely considered pathological hallmarks in Alzheimer’s disease (AD), Parkinson's disease (PD), and other neurodegenerative diseases. Therefore, the measurement of pathological Tau alterations including the level of phosphorylation and aggregation both serve as important biomarker predictions for the onset and progression for Alzheimer's disease. The LEGENDplex™ Human Phospho-Tau Panel (3-plex) is a bead-based multiplex assay, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 3 key phosphorylated Tau targets considered biomarkers in various neurodegenerative diseases including pTau217, pTau181, pTau262. This assay panel provides higher sensitivity and a broader dynamic range than traditional ELISA methods. The panel has been validated for use on human brain lysate, cerebrospinal fluid (CSF), cell culture supernatant, serum, and plasma.