Biolegend, 741580, LEGENDplex™ Mouse CD8/NK Panel (13-plex) w/ VbP

CD8⁺ cytotoxic T lymphocytes and natural killer (NK) cells are key immune effectors that eliminate virally infected and malignant cells through cytokine release and cytolytic mechanisms. In mice, they mediate cell death via the Fas/FasL pathway and granule exocytosis, releasing perforin and granzymes. Their activation also depends on co-stimulatory signals such as CD137 (4-1BB). Beyond cytotoxicity, they secrete cytokines— including IL-2, IFN-γ, TNF-α, IL-4, IL-6, IL-10, and IL-17A—that regulate inflammation and coordinate immune responses. These mechanisms collectively drive immune surveillance in murine models of infection, cancer, and autoimmunity. The LEGENDplex™ Mouse CD8/NK Panel is a bead-based multiplex assay, utilizing fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 mouse proteins, including IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF-α, sFas, sFasL, Granzyme A, Granzyme B, Perforin, and CD137 (4-1BB). This panel provides high sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant samples.