Biolegend, 752202, Recombinant Mouse KGF (FGF-7) (carrier-free)
10microg
Catalog Number: 752202
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Category
Pack Size107 units / pack
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| Source | Mouse KGF, amino acids Cys32-Thr194 (Accession# BC052847.1), was expressed in E. coli. |
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| Molecular Mass | The 163 amino acid recombinant protein has a predicted molecular mass of approximately 18.7 kD. The protein migrates at approximately 21 kD in DTT-reducing conditions and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Cys. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Sterile-filtered with 0.2 µm filter, solution is comprised of 10 mM MOPS, 50 mM Na2SO4, 0.5 mM EDTA, and 0.5 mM DTT in pH 7.2. |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | The ED50 is 0.1 - 0.5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 107 units/mg, as determined by a dose-dependent MCF-7 cell proliferation in the presence of 10 µg/ml heparin. The proliferation was measured by the colorimetric MTS assay. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Growth factor. |
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| Distribution | KGF is widely expressed among cells of mesenchymal origin. |
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| Function | KGF is secreted by fibroblasts and can stimulate the proliferation of epithelial cells. KGF expression can be highly upregulated in various tissues after injury. |
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| Interaction | Epithelial cells. |
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| Ligand/Receptor | FGF-7 receptor (FGFR2/IIIb). |
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| Cell Type | Embryonic Stem Cells |
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| Biology Area | Cell Proliferation and Viability, Stem Cells |
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| Molecular Family | Cytokines/Chemokines, Growth Factors |
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| Antigen References | 1. Werner S, et al. 1998. Cytokine Growth Factor Rev. 9:153. 2. Werner S, et al. 1994. Science 266:819. 3. Rossi SW, et al. 2007. Blood 109:3803. 4. Yen TT, et al. 2014. Protein Pept. Lett. 21:306. 5. Hsu YR, et al. 1999. Biochemistry 38:2523. |
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| Gene ID | 14178 |
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| UniProt | View information about KGF on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Keratinocyte growth factor, Fibroblast growth factor 7 (FGF-7), HBGF-7, FGF7, HBGF7 |
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