Biolegend, 752204, Recombinant Mouse KGF (FGF-7) (carrier-free)
25microg
Catalog Number: 752204
Send an RFQ.
Category
Pack Size107 units / pack
Loading…
| Source | Mouse KGF, amino acids Cys32-Thr194 (Accession# BC052847.1), was expressed in E. coli. |
|---|---|
| Molecular Mass | The 163 amino acid recombinant protein has a predicted molecular mass of approximately 18.7 kD. The protein migrates at approximately 21 kD in DTT-reducing conditions and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Cys. |
| Purity | >95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | Sterile-filtered with 0.2 µm filter, solution is comprised of 10 mM MOPS, 50 mM Na2SO4, 0.5 mM EDTA, and 0.5 mM DTT in pH 7.2. |
| Endotoxin Level | Less than 0.01 ng per µg cytokine as determined by the LAL method. |
| Concentration | 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | The ED50 is 0.1 - 0.5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 107 units/mg, as determined by a dose-dependent MCF-7 cell proliferation in the presence of 10 µg/ml heparin. The proliferation was measured by the colorimetric MTS assay. |
|---|
| Application | Bioassay |
|---|
| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
|---|
| Structure | Growth factor. |
|---|
| Distribution | KGF is widely expressed among cells of mesenchymal origin. |
|---|
| Function | KGF is secreted by fibroblasts and can stimulate the proliferation of epithelial cells. KGF expression can be highly upregulated in various tissues after injury. |
|---|
| Interaction | Epithelial cells. |
|---|
| Ligand/Receptor | FGF-7 receptor (FGFR2/IIIb). |
|---|
| Cell Type | Embryonic Stem Cells |
|---|
| Biology Area | Cell Proliferation and Viability, Stem Cells |
|---|
| Molecular Family | Cytokines/Chemokines, Growth Factors |
|---|
| Antigen References | 1. Werner S, et al. 1998. Cytokine Growth Factor Rev. 9:153. 2. Werner S, et al. 1994. Science 266:819. 3. Rossi SW, et al. 2007. Blood 109:3803. 4. Yen TT, et al. 2014. Protein Pept. Lett. 21:306. 5. Hsu YR, et al. 1999. Biochemistry 38:2523. |
|---|
| Gene ID | 14178 |
|---|
| UniProt | View information about KGF on UniProt.org |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Keratinocyte growth factor, Fibroblast growth factor 7 (FGF-7), HBGF-7, FGF7, HBGF7 |
|---|
