Biolegend, 753514, Cell-Vive™ GMP Recombinant Human Sonic Hedgehog (carrier-free)

Name: Cell-Vive™ GMP Recombinant Human Sonic Hedgehog (carrier-free)
Brand: Biolegend
SKU: 753514

25microg

Catalog Number: 753514

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Category

Amino Acids

Description

Human Sonic Hedgehog (SHH) encodes a 462 amino acid precursor protein. It undergoes autocatalytic internal cleavage to yield a non-glycosylated 19 kD N-terminal fragment, which has signaling activity, and a glycosylated 25 kD C-terminal protein that is active in precursor processing. The SHH N-terminal fragment is highly conserved between species with > 98% amino acid identity between human, mouse, and rat. Lipid modifications of the SHH N-terminal fragment enhances its binding affinity and modulates interactions with the Patched and Smoothened transmembrane receptors. SHH activity is attenuated by its interaction with membrane-associated Hip. On the other hand, interaction with glycan can lead to the stimulation of SHH signaling. SHH is expressed in embryonic tissues that are critical for the patterning of early embryos. It functions as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH regulates the differentiation and proliferation of early thymocyte progenitors and it also regulates the neural and hematopoietic stem cell fates. In adult tissues, SHH is associated with cancer development and tissue remodeling following injuries. Gastric SHH acts as a macrophage chemoattractant during the immune response to Helicobater pylori . BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

Specifications

General

Source	Human SHH, amino acids (Cys24-Gly197) (Cys24Ile-Ile) (Accession# NP_000184), with a N-terminal Met was expressed in E. coli.
Molecular Mass	The 176 amino acid recombinant protein has a predicted molecular mass of approximately 19.8 kD. The protein migrates at approximately 22 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.
N-terminalSequence Analysis	Met-Ile-Ile-Gly-Pro-Gly-Arg-Gly-Phe-Gly
Purity	≥ 95%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.1 μm filtered protein solution is in 10 mM sodium phosphate buffer, 0.3M NaCl, and in pH 7.2
Endotoxin Level	Less than or equal to 0.1 EU per μg of protein as determined by the LAL method.
Residual Host Cell Protein Content	≤ 0.500 ng/μg by ELISA
Concentration	25 μg & 100 μg sizes are bottled at 0.5 mg/mL
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Ordering

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Biolegend, 753514, Cell-Vive™ GMP Recombinant Human Sonic Hedgehog (carrier-free)

Send an RFQ.

General

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