Biolegend, 756224, Cell-Vive™ GMP Recombinant Human Cystatin C (carrier-free)
50microg
Catalog Number: 756224
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| Source | Human Cystatin C, amino acids Ser27-Ala146 (Accession# P01034) was expressed in 293E cells. The carboxy-terminal contains a TG-10His-GGQtag. |
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| Molecular Mass | The 135 amino acid recombinant protein has a predicted molecular mass of 15.1 kD. The non-reduced and DTT-reduced protein migrates at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Serine. |
| N-terminalSequence Analysis | Ser-Ser-Pro-Gly-Lys-Pro-Pro-Arg-Leu-Val |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | Protein was lyophilized from 0.1 μm filtered solution containing 20mM HEPES, 0.1M NaCl, pH7.2 |
| Endotoxin Level | Less than or equal to 0.1 EU per μg of protein as determined by LAL method. |
| Residual Host Cell Protein Content | ≤ 0.50 ng/μg by ELISA |
| Concentration | 50 μg and 500 μg sizes are lyophilized |
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| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile water. Before reconstitution, make sure sterile water and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile water) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/ thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months. |
| Activity | IC50 < 6.0 nM, as determined by inhibiting human Papain (0.05 μg/mL) activity when 100 µM of Z-FR-AMC is used as a papain peptide substrate. |
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| Application | Bioassay |
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| Application Notes | Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Structure | Monomer |
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| Function | Inhibitor of cysteine proteases (capthepsins B, H, K, L, and S). Transcription factor IFN regulatory factor 8 (IRF-8) induces cystatin C in mouse primary dendritic cells. IL-10 inhibits IRF-8 and consequently Cystatin C. |
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| Interaction | Normal and cancer cells. |
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| Ligand/Receptor | Papain-like cysteine proteases, cathepsins B, H, K, L, and S, and legumain. |
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| Bioactivity | Cystatin C inhibits the activity of papain. |
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| Cell Sources | Considered ubiquitously expressed, biological fluids, dendritic cells, and macrophages. |
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| Antigen References | Grubb A, et al. 1982. Proc. Nat. Acad. Sci. 79:3024. Janowski R, et al. 2001. Nat. Struct. Biol. 8:316. Sokol JP, Schiemann WP. 2004. Mol. Cancer Res. 2:183. Bird PI, et al. 2009. Nat Rev Immunol. 9:871. Kaur G, Levy E. 2012. Front Mol. Neurosci. 5:79. Xu Y, et al. 2014. J Biol Chem. 289:9730. Xu Y, et al. 2015. Immunol. Cell Biol. 5:442. |
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| Gene ID | 1471 |
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| UniProt | View information about Cystatin C on UniProt.org |
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| Other Names | Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, gamma-CSF, post-gamma protein |
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