Biolegend, 763714, Cell-Vive™ GMP Recombinant Human TPO (carrier-free)
25microg
Catalog Number: 763714
Send an RFQ.
Category
Loading…
| Source | This truncated Human TPO, (amino acids Ser22(Pro26del)-Gly353, Accession # NM_000460) was expressed in 293E cells. The C-terminal contains an -TG-8H-GGQ-tag. |
|---|---|
| Molecular Mass | The 344 amino acid recombinant protein has a predicted molecular mass of approximately 37 kD. The DTT-reduced and non-reduced proteins migrate to approximately 70 kDa by SDS-PAGE. The predicted N-terminal amino acid is Ser. |
| N-terminalSequence Analysis | Ser-Pro-Ala-Pro-Ala-(Cys)-Asp-(Leu)-Arg-Val |
| Purity | ≥ 95%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.1 µm filtered protein solution is in PBS, pH 7.2. |
| Endotoxin Level | Less than or equal to 0.1 EU per μg protein as determined by the LAL method |
| Residual Host Cell Protein Content | ≤ 0.500 ng/μg by ELISA |
| Concentration | 500 µg/mL |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Activity | ED50 = 0.15 - 0.6 ng/mL as measured by its ability to induce dose-dependent proliferation of MO7e megakaryocytic leukemia cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The specific activity of Cell-Vive™ GMP Recombinant Human TPO (carrier-free) is ≥1.5 x 106 IU/mg when compared against the WHO International Standard for Human TPO (NIBSC code: 03/124). |
|---|
| Application | Bioassay Cell Culture |
|---|
| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
|---|
| Disclaimer | BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
|---|
| Structure | Growth Factor |
|---|
| Distribution | TPO is produced by the liver and kidney and is expressed in the central nervous system and bone marrow. |
|---|
| Function | TPO regulates the megakaryocytopoiesis and thrombopoiesis |
|---|
| Interaction | Megakaryocytes, platelets, hemangioblasts, hematopoietic stem cells, and endothelial cells |
|---|
| Ligand/Receptor | c-Mpl |
|---|
| Bioactivity | Measured by its ability to induce proliferation of MO7e megakaryocytic leukemia cells. |
|---|
| Cell Type | Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells |
|---|
| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Stem Cells |
|---|
| Molecular Family | Cytokines/Chemokines, Growth Factors |
|---|
| Antigen References | Avanzi G, et al. 1988. Br J Haematol. 69:359. Deutsch V.R. and Tomer A. 2006. Br J Haematol. 134:453. Drachman JG, et al. 1995. J Biol Chem. 270:4979. Li J. et al. 1999. Br J Haematol. 106:345. Kato T, et al. 1997. Proc Natl Acad Sci. 94:4669. Ehrenreich H, et al. 2005. Proc Natl Acad Sci USA. 102:862. Royer Y, et al. 2005. J Biol Chem. 280:27251. Kaushansky K. 2005. J Clin Invest. 115:3339. de Graaf CA and Metcalf D. 2011. Cell Cycle. 10:1582. Zhang J, et al. 2010. J Interferon Cytokine Res. 7:465. Lupia E, et al. 2012. Mediators Inflamm. 2012:390892. Besancenot R, et al. 2014. Blood. 124:2104. |
|---|
| Gene ID | 7066 |
|---|
| UniProt | View information about TPO on UniProt.org |
|---|
| Other Names | Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus oncogene ligand (Mpl-ligand), Megakaryocyte growth and development factor (MGDF), THPO, Thrombopoietin, MPLLG, MGDF, ML |
|---|
